THERMOSTABLE REVERSE TRANSCRIPTASE AND USE THEREOF

The present invention relates to a thermostable reverse transcriptase and use thereof. The thermostable reverse transcriptase has at least one of mutation positions L333, P65, Q68, P127, P175, E176, G178, G331, T332, F334, and G105, wherein the amino acid positions of the thermostable reverse transc...

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Bibliographische Detailangaben
Hauptverfasser:	CAO, Ruyin, XIE, Qingqing, ZHANG, Xiaohong, CHEN, Xuemei, ZHENG, Yue, LIU, Xiaochen, DONG, Yuliang, ZHANG, Wenwei
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a thermostable reverse transcriptase and use thereof. The thermostable reverse transcriptase has at least one of mutation positions L333, P65, Q68, P127, P175, E176, G178, G331, T332, F334, and G105, wherein the amino acid positions of the thermostable reverse transcriptase are based on the amino acid sequence set forth in SEQ ID NO: 2. The reverse transcriptase has higher thermal stability. La présente invention concerne une transcriptase inverse thermostable et son utilisation. La transcriptase inverse thermostable présente au moins une des positions de mutation L333, P65, Q68, P127, P175, E176, G178, G331, T332, F334 et G105, les positions d'acides aminés de la transcriptase inverse thermostable étant fondées sur la séquence d'acides aminés représentée dans SEQ ID NO : 2. La transcriptase inverse présente une plus grande stabilité thermique. 本发明涉及一种热稳定逆转录酶及其应用。该热稳定逆转录酶具有L333、P65、Q68、P127、P175、E176、G178、G331、T332、F334和G105突变位点中的至少之一，其中，所述热稳定逆转录酶的氨基酸位点定位是以SEQ ID NO:2所示的氨基酸序列定