DETECTION METHOD FOR MONITORING SYNTHESIS OF RNA BY IN VITRO TRANSCRIPTION IN REAL TIME

A detection method for monitoring synthesis of RNA by in vitro transcription in real time, relating to the technical field of molecular biology and transcription. A DNA template suitable for in vitro transcription and a real-time fluorescent transcription system are developed, and a high-throughput,...

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Bibliographische Detailangaben
Hauptverfasser:	QIN, Weitong, LI, Liang, YANG, Fan, YANG, Guangyu
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A detection method for monitoring synthesis of RNA by in vitro transcription in real time, relating to the technical field of molecular biology and transcription. A DNA template suitable for in vitro transcription and a real-time fluorescent transcription system are developed, and a high-throughput, simple and sensitive RNA synthesis monitoring and quantification system is established. The DNA template/real-time fluorescent transcription system/monitoring system can be applied to synthesizing mRNA vaccines, screening RNA polymerases, analyzing promoter strength, screening drugs for inhibiting transcription, optimizing mRNA drug production process, training mRNA drug sequence AI design software and the like, and can further be applied to optimizing an in vitro transcription system, quantifying RNA polymerase activity, and optimizing sequences. Procédé de détection permettant de suivre en temps réel la synthèse de l'ARN par transcription in vitro, relatif au domaine technique de la biologie moléculaire et de la