NOVEL ANION-EXCHANGE CHROMATOGRAPHY METHODS FOR SEPARATION OF EMPTY FROM FULL RECOMBINANT ADENO-ASSOCIATED VIRUS PARTICLES

Provided are methods of separating full capsid particles and empty capsid particles in a viral capsid preparation by overloading viral capsid preparations onto an anion exchange medium integrated with weak partitioning chromatography and multiple column chromatography (AEX-WPC-MCC). Also provided ar...

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Bibliographische Detailangaben
Hauptverfasser:	DI, Wenjun, HUANG, Chao, WARREN, James, CAI, Ying, CHEN, Dennis Perry, MEDEIROS, Amy, KOCZERA, Kyle
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SEPARATION SPIRITS THEIR RELEVANT APPARATUS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided are methods of separating full capsid particles and empty capsid particles in a viral capsid preparation by overloading viral capsid preparations onto an anion exchange medium integrated with weak partitioning chromatography and multiple column chromatography (AEX-WPC-MCC). Also provided are viral capsid loading ranges for overloading AEX medium facilitating separating full and empty capsid particles. L'invention concerne des procédés de séparation de particules de capside pleines et de particules de capside vides dans une préparation de capside virale par surcharge de préparations de capside virale sur un milieu d'échange d'anions intégré avec une chromatographie de partitionnement faible et une chromatographie sur colonne multiple (AEX-WPC-MCC). L'invention concerne également des plages de chargement de capside virale pour surcharger un milieu AEX permettant la séparation de particules de capside pleines et vides.