DE NOVO PORES

Aspects of the disclosure relate to protein pore complexes and their uses in analyte detection and characterisation. The disclosure is based, in part, on nanopore complexes formed by CsgG-like pores and one or more auxiliary proteins, which form one or more channel constrictions in the nanopore comp...

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Bibliographische Detailangaben
Hauptverfasser:	MALAVIARACHCHIGE RABEL, Ranga Prabhath, DEGRADO, William F, SINGH, Pratik Raj, LECKENBY, Amber Elizabeth, RIERA, Alberto, JAYASINGHE, Lakmal Nishantha, HAMBLEY, Richard George, WALLACE, Elizabeth Jayne, SCOTT, Alistair James, GRIFFITHS, Rhys Connor, POLIZZI, Nicholas, SCHNAIDER, Lee
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MANUFACTURE OR TREATMENT OF NANOSTRUCTURES MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NANOTECHNOLOGY ORGANIC CHEMISTRY PEPTIDES PERFORMING OPERATIONS PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES SPIRITS TESTING TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Aspects of the disclosure relate to protein pore complexes and their uses in analyte detection and characterisation. The disclosure is based, in part, on nanopore complexes formed by CsgG-like pores and one or more auxiliary proteins, which form one or more channel constrictions in the nanopore complex. In some embodiments, the one or more auxiliary protein is a fusion protein. The disclosure further relates to methods for design of auxiliary proteins and production of the nanopore complexes, and for use in molecular sensing and analyte sequencing applications. Des aspects de la divulgation concernent des complexes poreux de protéines et leurs utilisations dans la détection et la caractérisation d'analytes. La divulgation se fonde, en partie, sur des complexes de nanopores constitués de pores de type CsgG et d'une ou plusieurs protéines auxiliaires, qui constituent une ou plusieurs constrictions de canal dans le complexe de nanopores. Dans certains modes de réalisation, la ou les protéines auxiliaires sont une protéine de fusion. L'invention concerne en outre des procédés de conception de protéines auxiliaires et de production des complexes de nanopores, et destinés à être utilisés dans des applications de détection moléculaire et de séquençage d'analytes.