RECOMBINATION SYSTEM FOR EXPRESSING FAB FRAGMENT LIBRARY

The present application discloses a plasmid combination for expressing an antibody Fab fragment library, containing a first plasmid and a second plasmid. The first plasmid contains an antibody heavy chain VH-CH1 expression unit and a first recombination site; the second plasmid contains an antibody...

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Bibliographische Detailangaben
Hauptverfasser:	WANG, Songmeng, LIU, Zhigang, HAO, Xiaobo, ZHOU, Xiaowei, LIU, Yulan, HUANG, Weiguo, LIU, Zibin
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present application discloses a plasmid combination for expressing an antibody Fab fragment library, containing a first plasmid and a second plasmid. The first plasmid contains an antibody heavy chain VH-CH1 expression unit and a first recombination site; the second plasmid contains an antibody light chain VL-CL expression unit and a second recombination site; and the first recombination site is different from the second recombination site. The present application provides a recombination system and recombinant cell for expressing an antibody Fab fragment library, and also provides a use of the plasmid combination, the recombination system and the recombinant cell, and a preparation method for an antibody Fab fragment library. La présente demande concerne une combinaison de plasmides pour exprimer une bibliothèque de fragments Fab d'anticorps, contenant un premier plasmide et un second plasmide. Le premier plasmide contient une unité d'expression VH-CH1 de chaîne lourde d'anticorps et un premier site de r