PAMAM/NANOPOROUS GOLD-MODIFIED SCREEN PRINTING CARBON ELECTRODE APTAMER BIOSENSOR, AND PREPARATION METHOD THEREFOR AND USE THEREOF

The present invention belongs to the technical field of biosensors. Provided in the present invention are a PAMAM/nanoporous gold-modified screen printing carbon electrode aptamer biosensor, and a preparation method therefor and the use thereof. In the present invention, mercaptoethylamine is loaded onto a nanoporous gold-modified screen printing electrode to form an Au-S bond, then PAMAM is fixed to the surface of the electrode using a glutaraldehyde cross-linking agent, and then the amino of PAMAM is linked to the amino in an ochratoxin A aptamer using a glutaraldehyde cross-linking agent so as to modify the surface of the PAMAM/NPG/SPCE sensor with the ochratoxin A aptamer. The biosensor can be used as a probe to achieve the specific detection of ochratoxin A. The PAMAM/nanoporous gold-modified screen printing carbon electrode aptamer biosensor provided by the present invention has the advantages of a strong specificity, a high sensitivity, etc., can be miniaturized, does not need professional operators to