METHOD AND APPARATUS USING ABSORPTION SPECTROSCOPY FOR DISCRIMINATION OF TISSUE OR CELLS
A method of discriminating between first cell types or first tissue types and second cell types or second tissue types is described. At S601, the method comprises providing a cell sample or a tissue sample including a first spatial region, comprising a first cell type or a first tissue type, and a s...
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Zusammenfassung: | A method of discriminating between first cell types or first tissue types and second cell types or second tissue types is described. At S601, the method comprises providing a cell sample or a tissue sample including a first spatial region, comprising a first cell type or a first tissue type, and a second spatial region. At S602, the method comprises obtaining a first absorption response comprising: illuminating the first spatial region using emitted infrared, IR, electromagnetic radiation, EMR, incident thereupon, wherein the IR EMR comprises a set of pairs of discrete wavelengths, including a first pair of discrete wavelengths consisting of a first wavelength and a second wavelength, wherein the first pair of discrete wavelengths is characteristic of a discriminating biomarker; and detecting respective absorbances of the incident first wavelength and the incident second wavelength of the first pair of discrete wavelengths by the first spatial region. At S603, the method comprises obtaining a second absorption response comprising: illuminating the second spatial region using the IR EMR; and detecting respective absorbances of the incident first wavelength and the incident second wavelength of the first pair of discrete wavelengths by the second spatial region. At S604, the method comprises discriminating between the first spatial region and the second spatial region based on a result of comparing the obtained first absorption response and the obtained second absorption response.
L'invention concerne une méthode de discrimination entre des premiers types de cellules ou des premiers types de tissu et des seconds types de cellules ou des seconds types de tissu. En S601, la méthode consiste à fournir un échantillon de cellule ou un échantillon de tissu comprenant une première région spatiale, comprenant un premier type de cellule ou un premier type de tissu, et une seconde région spatiale. En S602, la méthode consiste à obtenir une première réponse d'absorption comprenant : l'éclairage de la première région spatiale à l'aide d'un rayonnement électromagnétique (EMR) infrarouge (IR) émis incident sur cette dernière, l'EMR IR comprenant un ensemble de paires de longueurs d'onde discrètes, comprenant une première paire de longueurs d'onde discrètes constituée d'une première longueur d'onde et d'une seconde longueur d'onde, la première paire de longueurs d'onde discrètes étant caractéristique d'un biomarqueur de discrimination ; et la détection d'absorbances respec |
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