TRANSAMINASE MUTANT AND PREPARATION METHOD FOR CHIRAL AMINE COMPOUND

The present invention provides a transaminase mutant and a preparation method for a chiral amine compound. The transaminase mutant comprises: (a) a protein comprising an amino acid sequence represented by SEQ ID NO: 1; or (b) a protein comprising an amino acid sequence represented by SEQ ID NO: 2 or SEQ ID NO: 3; or (c) a protein that undergoes an amino acid mutation at at least one of the following sites of the amino acid sequence in (b): Y89, L380, N86, Y85, T91, P83, K90, S417, S424, F301, G164, T452, and the like, and has a transaminase function; and (d) a protein having homology of more than 80% to the amino acid sequence defined in any one of (a), (b) or (c) and having a transaminase function. The problem of low activity of transaminases in the prior art can be solved. The present invention is suitable for the field of enzyme catalysis. La présente invention concerne un mutant de transaminase et un procédé de préparation d'un composé amine chiral. Le mutant de transaminase comprend : (a) une protéine co