HELITRON COMPOSITIONS AND METHODS

The disclosure provides, e.g., compositions comprising a helitron tranposase or fragment or variant thereof, further comprising a gene modifying polypeptide or a nucleic acid encoding the gene modifying polypeptide, wherein the gene modifying polypeptide comprises: (i) a fragment of a helraiser transposase that lacks an N-terminal domain (ND), e.g., lacks both of an ND and a disordered domain (DD), or a sequence having defined identity thereto, and (ii) a DNA binding domain heterologous to (a)(1); and (b) a donor DNA molecule comprising a nucleic acid molecule comprising (i) one or both of: a right terminal sequence (RTS) and a left terminal sequence (ITS), and (ii) a heterologous object sequence and a heterologous DNA binding domain. Methods for making and using said compositions are also disclosed. L'invention concerne, par exemple, des compositions comprenant une transposase d'hélitron ou un fragment ou un variant de celle-ci, ainsi qu'un domaine de liaison à l'ADN hétérologue. L'invention concerne également des procédés associés.