METHODS OF INCREASING INTRACELLULAR TRANSLATIONAL EFFICIENCY AND/OR STABILITY OF RNA MOLECULES

A method for improving intracellular translational efficiency and/or stability of RNA molecules. An RNA molecule preparation method is provided, comprising a step of adding a modified poly (A) sequence composed of a modified adenylic acid and an optional unmodified adenylic acid at the 3' end o...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	WU, Ligang, LI, Shuoke, ZHANG, Hongdao
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	CHEMISTRY DERIVATIVES THEREOF HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES SUGARS
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A method for improving intracellular translational efficiency and/or stability of RNA molecules. An RNA molecule preparation method is provided, comprising a step of adding a modified poly (A) sequence composed of a modified adenylic acid and an optional unmodified adenylic acid at the 3' end of the poly (A) tail of a starting RNA molecule. The RNA molecule may be an mRNA molecule. Also provided are an RNA molecule, a pharmaceutical composition, a corresponding use, and a kit. The poly (A) tail of the RNA molecule prepared by the method is extended and has a modified adenylic acid at the extended portion. Compared with a starting RNA molecule without an extended poly (A) tail or an RNA molecule with an extended poly(A) tail that does not contain a modified adenylic acid, the present RNA molecule has improved intracellular translational efficiency and/or stability. La présente divulgation concerne un procédé d'amélioration de l'efficacité de traduction intracellulaire et/ou de la stabilité de molécules d'ARN.