PREPARATION METHOD AND USE OF AUTOLOGOUS TUMOR-DRAINING LYMPH NODE LYMPHOCYTES

The present invention provides a preparation method and the use of autologous tumor-draining lymph node lymphocytes. The preparation method comprises the following steps: A1, acquiring draining lymph node tissue from a patient, digesting same and separating same to prepare the draining lymph node mononuclear cells; and A2, culturing the cells obtained in the step A1, and activating and amplifying T cells contained in the cells to obtain the autologous tumor-draining lymph node lymphocytes. The preparation method of the present application can obtain the autologous tumor-draining lymph node lymphocytes (LNLs). Compared with tumor infiltrating lymphocytes, the LNLs as adoptive transfer cells facilitate in vitro culture amplification and increase the culture success rate. After adoptive transfer into a body, the LNLs can also provide a corresponding killing effect, and have excellent in-vivo amplification potential and in-vivo action durability and continuous activity, thus overcoming the defects of terminal dif