CELL CULTURE METHODS

A method of cell culture includes (i) culturing cells in a cell culture medium, and (ii) maintaining at least one metabolite below an inhibitory concentration in the cell culture medium for the at least one metabolite, wherein the at least one metabolite is aconitic acid (AA), leucinic acid (HICA),...

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Bibliographische Detailangaben
Hauptverfasser:	YOON, Seongkyu, HOANG, Duc, KUANG, Bingyu
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method of cell culture includes (i) culturing cells in a cell culture medium, and (ii) maintaining at least one metabolite below an inhibitory concentration in the cell culture medium for the at least one metabolite, wherein the at least one metabolite is aconitic acid (AA), leucinic acid (HICA), cytidine monophosphate (CMP), methylsuccinic acid (MSA), trigonelline (TRI), N-acetylputrescinium (NAP), or a combination thereof, and wherein the enzyme comprises ADH5, BCAT1, CAT, GOT1, HADHB, H0GA1, SLC35A1, or a combination thereof. Un procédé de culture cellulaire comprend (i) la culture de cellules dans un milieu de culture cellulaire, et (ii) le maintien d'au moins un métabolite au-dessous d'une concentration inhibitrice dans le milieu de culture cellulaire pour le ou les métabolites, le ou les métabolites étant l'acide aconitique (AA), l'acide leucinique (HICA), le monophosphate de cytidine (CMP), l'acide méthylsuccinique (MSA), la trigonelline (TRI), le N-acétylputrescinium (NAP), ou une combinaison de ceux-ci, et l'enzyme comprenant ADH5, BCAT1, CAT, GOT1, HADHB, H0GA1, SLC35A1, ou une combinaison de ceux-ci.