SYSTEMS AND METHODS OF DETECTING MERGED DROPLETS IN SINGLE CELL SEQUENCING

Disclosed herein are methods for detecting one or more droplet mergers in a single cell sequencing workflow, including obtaining a dataset comprising sequence reads of a plurality of amplicons comprising barcodes associated with a plurality of cells; normalizing the dataset; clustering the barcodes;...

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Bibliographische Detailangaben
Hauptverfasser:	SCIAMBI, Adam, PARIKH, Saurabh, PARIKH, Anup
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed herein are methods for detecting one or more droplet mergers in a single cell sequencing workflow, including obtaining a dataset comprising sequence reads of a plurality of amplicons comprising barcodes associated with a plurality of cells; normalizing the dataset; clustering the barcodes; and determining the one or more droplet mergers by labelling one or more clusters. La présente invention concerne des procédés pour : détecter une ou plusieurs fusions de gouttelettes dans un flux de travail de séquençage monocellulaire, y compris l'obtention d'un ensemble de données comprenant des lectures de séquence d'une pluralité d'amplicons comprenant des codes-barres associés à une pluralité de cellules ; normaliser l'ensemble de données ; regrouper les codes-barres ; et déterminer un ou plusieurs éléments de fusion de gouttelettes en marquant un ou plusieurs amas.