METHOD FOR OBTAINING INFORMATION ON NANOANTIBODY SPECIFICALLY RECOGNIZING ANTIGEN

Provided is a method for obtaining information on a nanobody specifically recognizing an antigen, comprising: enriching B cells from peripheral blood lymphocytes of a subject; using a microfluidic droplet generation chip to wrap a solution containing an antigen and a signal detection reagent and a s...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	LIU, Xiaopan, WANG, Shiyu, REN, Bingzhao, ZHENG, Yue, LIU, Ya, LIU, Longqi, LIANG, Yuanqing, SHEN, Mengzhe, LV, Menghua, SUN, Na, LIU, Yang, GAO, Kai, WANG, Meiniang
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	CHEMISTRY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY ORGANIC CHEMISTRY PEPTIDES PHYSICS TESTING
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Provided is a method for obtaining information on a nanobody specifically recognizing an antigen, comprising: enriching B cells from peripheral blood lymphocytes of a subject; using a microfluidic droplet generation chip to wrap a solution containing an antigen and a signal detection reagent and a single B cell in a single droplet; incubating the generated single droplet, such that the B cell secretes a nanoantibody and the antigen is specifically bound to the nanoantibody; sorting the incubated droplets by using a droplet sorting chip, analyzing collected detectable signals so as to sort out a target cell capable of secreting a target nanobody, and collecting a positive droplet containing the target cell; and performing single cell sequencing on the target cell to obtain target nanoantibody information. L'invention concerne un procédé pour obtenir des informations sur un nano-corps reconnaissant spécifiquement un antigène, consistant : à enrichir des lymphocytes B à partir de lymphocytes de sang périphérique