ACTIVATED LYMPHOCYTE EXPANSION METHOD HAVING STABLE AND CONTROLLABLE QUALITY, AND USE THEREOF FOR PREVENTION AND CONTROL OF NEUROLOGICAL DISEASE

The present invention relates to a method for preparing an activated expanded lymphocyte stock solution, the preparation method comprising the following steps: (1) In a serum-free medium, co-culturing a lymphocyte activator with peripheral blood mononuclear cells (PBMCs) obtained via autologous peripheral whole blood centrifugal extraction, and thus completing lymphocyte culture activation, the serum-free medium being selected from any one of or combination of KBM 581 and GT-T551 H3; (2) placing the cultured activated lymphocytes prepared in step (1) into a serum-free medium, and performing subculturing to prepare a lymphocyte activation culture; (3) adding to the lymphocyte activation culture obtained via the subculturing in step (2) a serum-free medium having a volume which is 5-10 times thereof, and performing expansion culture, wherein the number of expansion culture generations is 1-5; (4) centrifuging, washing, and collecting the activated expanded lymphocytes. The activated expanded lymphocyte prepared