COMPOSITE REAGENT FOR PREPARING ULTRA-HIGH-RESOLUTION CHROMOSOMES AND USE METHOD THEREOF
A composite reagent for preparing ultra-high-resolution chromosomes and the use method thereof. The composite reagent is composed of the following components: 0.1 μmol/L-0.1 mmol/L RO-3306, 2 nmol/L-2 μmol/L TSA, and DMSO as a solvent, and named an HD850 reagent. The composite reagent is used for pr...
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| creator | LI, Nenggan LIU, Hengtao LIU, Jiaen LI, Cunxi |
| description | A composite reagent for preparing ultra-high-resolution chromosomes and the use method thereof. The composite reagent is composed of the following components: 0.1 μmol/L-0.1 mmol/L RO-3306, 2 nmol/L-2 μmol/L TSA, and DMSO as a solvent, and named an HD850 reagent. The composite reagent is used for preparing ultra-high-resolution chromosomes of peripheral blood cells, stem cells and other culture cells, which enable the proportion of ultra-high-resolution chromosomes to be significantly high, wherein 550 bands per haploid set can be obtained for 30% or more of metaphase cells, 700 bands per haploid set can be obtained for 10% or more of metaphase cells, and 850 or more bands per haploid set can be obtained for 20% or more of metaphase cells. The composite reagent does not contain a teratogenic and carcinogenic reagent, has the advantages of no harm to laboratory personnel, no environmental pollution, simple operation, high speed and high efficiency, solves the market gap for urgently needed composite reagents f |
| format | Patent |
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The composite reagent is composed of the following components: 0.1 μmol/L-0.1 mmol/L RO-3306, 2 nmol/L-2 μmol/L TSA, and DMSO as a solvent, and named an HD850 reagent. The composite reagent is used for preparing ultra-high-resolution chromosomes of peripheral blood cells, stem cells and other culture cells, which enable the proportion of ultra-high-resolution chromosomes to be significantly high, wherein 550 bands per haploid set can be obtained for 30% or more of metaphase cells, 700 bands per haploid set can be obtained for 10% or more of metaphase cells, and 850 or more bands per haploid set can be obtained for 20% or more of metaphase cells. 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The composite reagent is composed of the following components: 0.1 μmol/L-0.1 mmol/L RO-3306, 2 nmol/L-2 μmol/L TSA, and DMSO as a solvent, and named an HD850 reagent. The composite reagent is used for preparing ultra-high-resolution chromosomes of peripheral blood cells, stem cells and other culture cells, which enable the proportion of ultra-high-resolution chromosomes to be significantly high, wherein 550 bands per haploid set can be obtained for 30% or more of metaphase cells, 700 bands per haploid set can be obtained for 10% or more of metaphase cells, and 850 or more bands per haploid set can be obtained for 20% or more of metaphase cells. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE |
| title | COMPOSITE REAGENT FOR PREPARING ULTRA-HIGH-RESOLUTION CHROMOSOMES AND USE METHOD THEREOF |
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