ANCESTRAL SEQUENCE RECONSTRUCTION-BASED ω-TRANSAMINASE MUTANT

Provided is an ancestral sequence reconstruction-based ω-transaminase mutant from Aspergillus terreus. The amino acid sequence of the mutant is as shown in SEQ ID NO: 4 or SEQ ID NO: 6. Compared with a wild-type enzyme, the thermal stability of the ω-transaminase mutant is improved. Specifically, th...

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Bibliographische Detailangaben
Hauptverfasser:	CAO, Jiaren, HUANG, Jun, QIU, Shuai, LI, Yuanyuan, ZHAO, Weirui, LYU, Changjiang, FAN, Fangfang, WANG, Feng, HU, Sheng, LI, Ye, CAI, Tingting, MEI, Lehe
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided is an ancestral sequence reconstruction-based ω-transaminase mutant from Aspergillus terreus. The amino acid sequence of the mutant is as shown in SEQ ID NO: 4 or SEQ ID NO: 6. Compared with a wild-type enzyme, the thermal stability of the ω-transaminase mutant is improved. Specifically, the half-life of the mutant is 24 h or above, and the half-life of the wild-type enzyme is 6.90 min. The semi-inactivation temperature of the mutant is about 49.00°C, and the semi-inactivation temperature of the wild-type enzyme is 37.89°C. La présente invention concerne un mutant de ω-transaminase d'Aspergillus terreus fondé sur la reconstruction de séquences ancestrales. La séquence d'acides aminés du mutant est telle que représentée dans SEQ ID NO : 4 ou SEQ ID NO : 6. Par comparaison avec une enzyme de type sauvage, la stabilité thermique du mutant ω-transaminase est améliorée. Plus particulièrement, la demi-vie du mutant est de 24 h ou plus, et la demi-vie de l'enzyme de type sauvage est de 6,90 min. La températ