RECOMBINANT COLLAGEN, PREPARATION METHOD THEREFOR AND APPLICATION THEREOF
The present invention relates to recombinant collagen, a preparation method therefor and an application thereof, and in particular, to a recombinantly expressed full-length collagen α1 chain, a preparation method therefor and an application thereof, relating to the technical field of collagen expres...
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Zusammenfassung: | The present invention relates to recombinant collagen, a preparation method therefor and an application thereof, and in particular, to a recombinantly expressed full-length collagen α1 chain, a preparation method therefor and an application thereof, relating to the technical field of collagen expression. When a human I-type collagen α1 chain (α1(I) chain) variant (recorded as α1(I)M1) and a human II-type collagen α1 chain (α1(II) chain) variant (recorded as α1(II)M6) constructed in the present invention are recombinantly expressed in Pichia pastoris, main degradation products (main degradation bands) which are basically the same as full-length α1 chain target products (target bands) in proportion and appear during recombinant expression of natural full-length α1(I) chain and α1(II) chain are eliminated, so that the yield of the target products is increased, and compared with natural full-length α1(I) chain collagen and α1(II) chain collagen recombinantly expressed in Pichia pastoris, α1(Ⅰ)M1 and α1(Ⅱ)M6 have |
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