OPTICAL PATH DESIGN METHOD AND DEVICE FOR FLUORESCENCE DISPERSION OF FLOW CYTOMETER

An optical path design method and device for fluorescence dispersion of a flow cytometer, relating to the technical field of flow cytometers. The optical path design method for fluorescence dispersion of a flow cytometer comprises: determining total light transmittance required and material parameters of prisms used (110); establishing a prism dispersion model, the prism dispersion model determining, according to the total light transmittance and the material parameters of the prisms used, an optical path shape when the total dispersion rate value is the largest when light continuously enters at least one prism and then reaches a receiving screen (120); and determining the total path length of the optical path, and determining the size of the optical path shape according to the total path length of the optical path (130). According to the method and device, a shading slit required when light is incident in a grating dispersion system can be omitted, the utilization rate, the signal-to-noise ratio and the sens