SMART CELL FACTORY OF HIGH VALUE-ADDED METABOLITE CONSTRUCTED ON BASIS OF AUTOMATION TECHNOLOGY

A smart cell factory of a high value-added metabolite constructed on the basis of automation technology, which belongs to the technical field of metabolic engineering. According to the present invention, a key enzyme mutant gene with a high efficiency is screened out from an iterative key enzyme gene mutation library by means of weakening the expression of the key enzyme gene of the high value-added metabolite, using the automation technology, and in combination with a mutation-screening-mutation closed-loop protein evolution method. Further provided are a method for constructing the smart cell factory of the high value-added metabolite and the use thereof. According to the present invention, the problem that there is currently no high-throughput molecular probe for the high value-added metabolite or the molecular probe screening thereof is unstable can be effectively solved, and labor costs are reduced, the protein evolution time is shortened, the inhibitory effects of an intermediate product and a final pro