NUCLEIC ACID-METHYLATED CYTOSINE CONVERSION METHOD

The present application provides a nucleic acid-methylated cytosine conversion method, comprising steps: (1) performing a TET enzyme oxidation treatment on a DNA or RNA sample; (2) adding pyridine borane into a reaction system for treatment; and (3) recovering DNA or RNA in the reaction system by me...

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Bibliographische Detailangaben
Hauptverfasser:	LIU, Qian, WANG, Man, SONG, Dongliang, CAO, Zhen, CHEN, Jingjing, HOU, Ce
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present application provides a nucleic acid-methylated cytosine conversion method, comprising steps: (1) performing a TET enzyme oxidation treatment on a DNA or RNA sample; (2) adding pyridine borane into a reaction system for treatment; and (3) recovering DNA or RNA in the reaction system by means of magnetic beads. The entire process of the nucleic acid-methylated cytosine conversion method of the present application only comprises three steps, i.e., TET enzyme oxidation, treatment with pyridine borane, and DNA recovery by means of magnetic beads, such that the conversion efficiency of TAPS is improved, and the operation difficulty and the operation flow of the TAPS are also simplified. The nucleic acid-methylated cytosine conversion method is named as Enhanced TET-assisted pyridine borane sequencing (eTAPS). La présente invention fournit un procédé de conversion de cytosine méthylée d'acide nucléique, comprenant les étapes suivantes : (1) réalisation d'un traitement d'oxydation par l'enzyme TET sur un