T7 RNA POLYMERASE VARIANTS FOR RNA SYNTHESIS

The present disclosure provides T7 RNA polymerase variants, the use of which increases transcription efficiency and improves the quality and yield of the enzyme. Also provided are polynucleotides encoding the T7 RNA polymerase variants, host cells capable of expressing the T7 RNA polymerase variants...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	RUDRAPPA, Deepak, DORR, Brent M, MELBY, Joel, VAIDYA, Nilesh, JELIAZKOV, Jeliazko
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present disclosure provides T7 RNA polymerase variants, the use of which increases transcription efficiency and improves the quality and yield of the enzyme. Also provided are polynucleotides encoding the T7 RNA polymerase variants, host cells capable of expressing the T7 RNA polymerase variants, and methods of using the T7 RNA polymerase variants for high-efficiency transcription. La présente invention concerne des variants d'ARN polymérase T7, l'utilisation de ceux-ci augmentant l'efficacité de transcription et améliorant la qualité et le rendement de l'enzyme. L'invention concerne également des polynucléotides codant pour les variants d'ARN polymérase T7, des cellules hôtes capables d'exprimer les variants d'ARN polymérase T7, ainsi que des procédés d'utilisation des variants d'ARN polymérase T7 pour une transcription à haut rendement.