ANION-EXCHANGE CHROMATOGRAPHY METHODS FOR PURIFICATION OF RECOMBINANT ADENO-ASSOCIATED VIRUSES

Provided are methods of separating full capsid particles and empty capsid particles in a viral capsid preparation using an anion exchange medium. Methods may involve using a wash solution comprising a quaternary ammonium salt. Methods include applying a viral capsid preparation to an anion exchange...

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Bibliographische Detailangaben
Hauptverfasser:	DI, Wenjun, HUANG, Chao, CAI, Ying, CHEN, Dennis Perry, WARREN, James C
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SEPARATION SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided are methods of separating full capsid particles and empty capsid particles in a viral capsid preparation using an anion exchange medium. Methods may involve using a wash solution comprising a quaternary ammonium salt. Methods include applying a viral capsid preparation to an anion exchange medium, combined with multiple quaternary ammonium salt wash solutions. L'invention concerne des procédés de séparation de particules de capside pleines et de particules de capside vides dans une préparation de capside virale à l'aide d'un milieu d'échange d'anions. Les procédés peuvent impliquer l'utilisation d'une solution de lavage comprenant un sel d'ammonium quaternaire. Les procédés comprennent l'application d'une préparation de capside virale à un milieu d'échange d'anions, combiné à de multiples solutions de lavage de sel d'ammonium quaternaire.