IMINE REDUCTASE MUTANT, CO-EXPRESSED ENZYME OF IMINE REDUCTASE AND GLUCOSE DEHYDROGENASE, AND APPLICATION THEREOF

Disclosed in the present invention are an imine reductase mutant, and a method for preparing (S)-nicotine using the imine reductase mutant. The first objective of the invention is to provide an imine reductase mutant, for solving the problems in the prior art that no nicotine of S configuration can...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
1. Verfasser: CHEN, Zecong
Format: Patent
Sprache:chi ; eng ; fre
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Disclosed in the present invention are an imine reductase mutant, and a method for preparing (S)-nicotine using the imine reductase mutant. The first objective of the invention is to provide an imine reductase mutant, for solving the problems in the prior art that no nicotine of S configuration can be obtained by using an enzyme and the enzyme activity is low, and this mutant can implement catalytic reduction of a substrate myosmine or 4-(Methylamino)-1-(pyridin-3-yl)butanone to (S)-nicotine. The second objective of the present invention is to provide a co-expressed enzyme of the imine reductase and a glucose dehydrogenase, specifically being a co-expressed enzyme of an imine reductase mutant enzyme and the glucose dehydrogenase. The co-expressed enzyme is used to prepare (S)-nicotine, and the cost of enzymatically preparing (S)-nicotine is lowered. La présente invention concerne un mutant d'imine réductase, et un procédé de préparation de (S)-nicotine utilisant le mutant d'imine réductase. Le premier objecti