METHOD FOR IMMUNOSENSING ON A LIPID LAYER USING MAGNETIC TUNNEL JUNCTIONS

The present invention relates to diagnostic test and technology. In particular, the present invention relates to a method for determining an analyte suspected to be present in a sample comprising contacting said sample with a sensor element comprising an anchor layer which is present on a solid support, a first binding agent which is capable of specifically binding to the analyte, which is anchored in the anchor layer and which comprises at least one magnetic label, wherein said at least one magnetic label is located within the anchor layer, a second binding agent which is capable of specifically binding to the analyte when bound to the first binding agent and which is immobilized on the solid support, and a magnetic tunnel junction in functional proximity to the second binding agent which generates a signal elicited in proximity to the at least one magnetic label of the first binding agent, for a time and under conditions which allow for specific binding of the analyte suspected to be present in the sample to the first binding agent and specific binding of the second binding agent to the analyte bound to the first binding agent and detecting the formation the complex of first binding agent, analyte and second binding agent based on the signal which is generated by the magnetic tunnel junction whereby the analyte is determined. Moreover, provided is a device for determining an analyte suspected to be present in a sample and the use thereof for determining an analyte suspected to be present in a sample in said sample. Moreover, the present invention contemplates a kit for determining an analyte suspected to be present in a sample. La présente invention concerne la technologie et le test de diagnostic. En particulier, la présente invention concerne un procédé de détermination d'un analyte suspecté d'être présent dans un échantillon qui consiste à mettre en contact ledit échantillon avec un élément capteur comprenant une couche d'ancrage qui est présente sur un support solide, un premier agent de liaison qui est apte à se lier spécifiquement à l'analyte, qui est ancré dans la couche d'ancrage et qui comprend au moins une étiquette magnétique, ladite au moins une étiquette magnétique étant située à l'intérieur de la couche d'ancrage, un second agent de liaison qui est apte à se lier spécifiquement à l'analyte lorsqu'il est lié au premier agent de liaison et qui est immobilisé sur le support solide et une jonction tunnel magnétique à proximité fonctionnelle du