FLOW DEPROTEINIZER AND A METHOD OF DEPROTEINIZATION OF LIQUID BIOLOGICAL SAMPLES

The flow deproteinizer, comprising the line for the liquid sample containing the protein, the deproteinization chamber and the line for discharging the liquid deproteinized sample, is characterized by the fact that it operates in a precipitation mode and is equipped with a vibration system ensuring the possibility of shaking the contents of the deproteinization chamber (2) which has a volume of 1.2-1.8 cm3, preferably 1.5 cm3, and the shape of a prism or inverted cartridge oriented in the vertical axis, the height of which is greater than the diagonal of the base, thanks to which it is possible to easily spontaneously separate the deproteinized fraction from the fraction containing the protein precipitate, whereby the deproteinizer is connected to the analytical system by means of flexible lines enabling it to vibrate, and it also includes a deproteinized sample drainage system, a protein-containing precipitate drainage system, a deproteinization chamber cleaning system and a venting system, whereby all inlet and outlet channels are equipped with electronically controlled valves and, preferably, an optical system for measuring the turbidity of the deproteinized sample. The method of deproteinizing liquid biological samples using a protein denaturation reaction with a precipitating agent is characterized in that the denaturation reaction of a protein contained in a biological sample is initiated by introducing a precipitation agent and carried out in the flow deproteinizer described above. The flow deproteinizer which enables the deproteinization of biological samples in the flow-through mode, allows to shorten the analysis time and simplify the procedure of sample preparation in relation to the deproteinization performed in the manual mode, as well as to reduce the costs of analysis and operation of the system in relation to the deproteinization in the flow-through mode with membranes. L'invention concerne un déprotéiniseur d'écoulement, comprenant une conduite pour un échantillon liquide contenant une protéine, une chambre de déprotéinisation et une conduite d'évacuation d'un échantillon déprotéiné liquide, qui est caractérisé en ce qu'il fonctionne dans le mode de précipitation et qu'il est équipé d'un système de vibration assurant la possibilité d'agiter le contenu de la chambre de déprotéinisation (2) qui possède un volume de 1,2 à 1,8 cm3, de préférence de 1,5 cm3, et qu'il possède la forme d'un prisme ou d'une cartouche inversée orientée selon l'axe