CELL BARCODE MICROBEAD CONTAINING FRAMESHIFT BASE, PREPARATION METHOD THEREFOR AND APPLICATION THEREOF

Provided are a cell barcode microbead containing a frameshift base, a preparation method therefor and an application thereof. The cell barcode microbead comprises a microbead body and a nucleic acid sequence structure marked on the body and having a cell barcode sequence. Frameshift base sequences h...

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Bibliographische Detailangaben
Hauptverfasser:	GUAN, Ying, SANG, Guoqin, HAN, Jinhuan, JIAO, Shaozhuo, LUO, Yunchao, MA, Xingyong, LIU, Shujie
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided are a cell barcode microbead containing a frameshift base, a preparation method therefor and an application thereof. The cell barcode microbead comprises a microbead body and a nucleic acid sequence structure marked on the body and having a cell barcode sequence. Frameshift base sequences having different lengths of different types of cell barcode microbeads are inserted at 5' terminals of cell barcode sequences, such that the base distribution balance of a single-cell sequencing library during sequencing can be realized, thereby improving sequencing quality and reducing sequencing cost. Microbille à code-barres cellulaire contenant une base de décalage de cadre de lecture, son procédé de préparation et son application. La microbille à code-barres cellulaire comprend un corps de microbille et une structure de séquence d'acide nucléique marquée sur le corps et ayant une séquence à code-barres cellulaire. Des séquences de bases à décalage de cadre de lecture ayant des longueurs différentes de différent