ANTIBODIES SPECIFIC FOR ALPHA-1,6-CORE-FUCOSYLATED PSA AND FUCOSYLATED FRAGMENTS THEREOF

The present invention relates to an antibody or an antigen binding fragment thereof that specifically binds to α- 1,6-core-fucosylated prostate specific antigen (PSA) and partial sequences thereof comprising the α-1,6-core-fucose residue. The antibodies and antigen binding fragments significantly discriminate between core-fucosylated PSA or core- fucosylated PSA partial sequences and other glycosylated PSA species and partial sequences thereof lacking the core-fucose residue, including aglycosylated PSA, as well as core- fucosylated glycan in other contexts. The present invention further relates to nucleic acid molecules encoding the light chain variable region or the heavy chain variable region of the antibody of the invention, as well as vectors comprising said nucleic acid molecules. The invention also relates to a host cell comprising the vector(s) of the invention, as well as to methods for the production of an antibody or antigen binding fragment of the invention comprising culturing the host cell of the invention under suitable conditions and isolating the antibody produced. Furthermore, the present invention relates to an antibody obtainable by the method of the invention, to a composition comprising at least one of the antibody or antigen binding fragment of the invention, the nucleic acid molecule of the invention, the vector of the invention, the host cell of the invention or the antibody produced by the method of the invention. The present invention also relates to the use of an antibody or antigen binding fragment of the invention for detecting and discriminating core-fucosylated PSA or core-fucosylated partial sequences thereof in biological samples. La présente invention concerne un anticorps ou un fragment de liaison à l'antigène de celui-ci qui se lie de manière spécifique à un antigène spécifique de la prostate α-1,6-noyau-fucosylé (PSA) et des séquences partielles de celui-ci notamment le résidu α-1,6-noyau-fucose. Les anticorps et les fragments de liaison à l'antigène différencient significativement entre PSA noyau-fucosylé ou des séquences partielles de PSA noyau-fucosylé et autres espèces de PSA glycosylé et des séquences partielles de celui-ci dépourvues du résidu noyau-fucose, y compris PSA aglycosylé, ainsi qu'un glycane noyau-fucosylé dans d'autres contextes. La présente invention concerne en outre des molécules d'acide nucléique codant pour la région variable de chaîne légère ou la région variable de chaîne lourde de l'anticorps