MASSIVELY PARALLEL ENZYMATIC SYNTHESIS OF POLYNUCLEOTIDES

The invention is directed to methods and compositions for inkjet assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions of the invention include formulations of synthe...

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Bibliographische Detailangaben
Hauptverfasser:	LACHAIZE, Henri, VERARDO, Damiano, HORGAN, Adrian, GODRON, Xavier
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	ADHESIVES BEER BIOCHEMISTRY CHEMICAL PAINT OR INK REMOVERS CHEMISTRY COATING COMPOSITIONS, e.g. PAINTS, VARNISHES ORLACQUERS COLOUR PRINTING COMPOSITIONS THEREOF CORRECTING FLUIDS CULTURE MEDIA DYES ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FILLING PASTES INKS LINING MACHINES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MISCELLANEOUS APPLICATIONS OF MATERIALS MISCELLANEOUS COMPOSITIONS MUTATION OR GENETIC ENGINEERING NATURAL RESINS PAINTS PASTES OR SOLIDS FOR COLOURING OR PRINTING PERFORMING OPERATIONS POLISHES PRINTING PRINTING, DUPLICATING, MARKING, OR COPYING PROCESSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS STAMPS TRANSPORTING TYPEWRITERS USE OF MATERIALS THEREFOR VINEGAR WINE WOODSTAINS
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Beschreibung
Zusammenfassung:	The invention is directed to methods and compositions for inkjet assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions of the invention include formulations of synthesis reagents for inkjet delivery including, but not limited to, TdT coupling reaction buffers and 3'-O-protected dNTP monomers. L'invention concerne des procédés et des compositions pour la synthèse assistée par jet d'encre d'une pluralité de polynucléotides au niveau de sites de réaction sur un substrat à l'aide de polymérases sans matrice, telles que des désoxynucléotidyltransférases terminales (TdT). Les compositions de l'invention comprennent des formulations de réactifs de synthèse pour l'administration par jet d'encre comprenant, non limitativement, des tampons de réaction de couplage de TdT et des monomères dNTP 3'-O-protégés.