URATE OXIDASE-ALBUMIN CONJUGATE HAVING CERTAIN NUMBER OF CONJUGATED ALBUMINS, AND METHOD FOR PRODUCING SAME

The present application relates to a method for producing urate oxidase containing non-natural amino acids, and urate oxidase produced thereby. It was confirmed that the method for producing urate oxidase containing non-natural amino acids disclosed herein allows drug delivery systems to be efficien...

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Bibliographische Detailangaben
Hauptverfasser: KWON, In Chan, PARK, Jun Yong, YANG, Byung Seop, LEE, Ja Yeon, CHO, Jeong Haeng, KIM, Hyun Woo
Format: Patent
Sprache:eng ; fre ; kor
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Zusammenfassung:The present application relates to a method for producing urate oxidase containing non-natural amino acids, and urate oxidase produced thereby. It was confirmed that the method for producing urate oxidase containing non-natural amino acids disclosed herein allows drug delivery systems to be efficiently connected to proteins, and thus can be effectively used to increase the half-life of proteins to which connecting drug delivery systems was difficult. In addition, the urate oxidase produced by the method is characterized in that a drug delivery system is selectively connected to a specific site such that the efficacy of the urate oxidase is maintained, the drug persistence increases, the risk of immune response decreases, and a uniform conjugate is generated so as to be easy to isolate. Therefore, the urate oxidase can be usefully applied to various biodrugs. La présente demande concerne un procédé de production d'urate oxydase contenant des acides aminés non naturels, et l'urate oxydase ainsi produite. Il a été confirmé que le procédé de production d'urate oxydase contenant des acides aminés non naturels divulgué ici permet de lier efficacement des systèmes d'administration de médicament à des protéines, et peut ainsi être efficacement utilisé pour augmenter la demi-vie de protéines pour lesquelles la liaison à des systèmes d'administration de médicament était difficile. De plus, l'urate oxydase produite par le procédé est caractérisée en ce qu'un système d'administration de médicament est sélectivement lié à un site spécifique de sorte que l'efficacité de l'urate oxydase soit maintenue, la persistance du médicament augmente, le risque de réponse immunitaire diminue, et un conjugué uniforme est généré afin d'être facile à isoler. Par conséquent, l'urate oxydase peut être mise à profit dans divers biomédicaments. 본 출원은 비천연아미노산을 포함하는 요산산화효소를 제조하는 방법 및 이에 따라 제조된 요산산화효소에 관한 것이다. 본 출원에서 비천연아미노산을 포함하는 요산산화효소를 제조하는 방법이 단백질에 약물전달체가 효율적으로 연결되어, 약물전달체를 연결하기 어려웠던 단백질의 반감기를 향상시키는데 효과적으로 이용될 수 있음을 확인하였다. 또한, 상기 방법에 의하여 생산된 요산산화효소는 특정위치에 선택적으로 약물전달체가 결합되어 약효가 유지되고 약물 지속성이 증가되며 면역반응 위험성이 감소하고, 균일한 복합체 생성으로 분리에 용이하여 다양한 바이오 의약품 등에 유용하게 활용될 수 있다.