REDUCTASE ENZYMES AND PROCESSES FOR MAKING AND USING REDUCTASE ENZYMES

The present disclosure provides ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing N-(7-((2S,4R,5R)-4-fluoro-3,3-dihydroxy-5-(hydroxymethyl) tetrahydrofuran-2-yl)-4-oxo-4,7-dihydro-3H-pyrrolo[3,2-d...

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Bibliographische Detailangaben
Hauptverfasser:	PAN, Weilan, XIAO, Li, VERMA, Deeptak, MCINTOSH, John, BENKOVICS, Tamas, MOORE, Jeffrey, CAMACHO SOTO, Karla
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present disclosure provides ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing N-(7-((2S,4R,5R)-4-fluoro-3,3-dihydroxy-5-(hydroxymethyl) tetrahydrofuran-2-yl)-4-oxo-4,7-dihydro-3H-pyrrolo[3,2-d]pyrimidin-2-yl)isobutyramide. Also provided are polynucleotides encoding the ketoreductase enzymes, host cells capable of expressing the ketoreductase enzymes, and methods of using the ketoreductase enzymes to synthesize N-(9-((2R,3S,4S,5R)-4-fluoro-3-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-6-oxo-6,9-dihydro-1H-purin-2-yl)isobutyramide. La présente invention concerne des enzymes cétoréductases ayant des propriétés améliorées par comparaison avec une enzyme cétoréductase de type sauvage naturelle ayant la capacité de réduire le N-(7-((2S,4R,5R)-4-fluoro-3,3-dihydroxy-5-(hydroxyméthyl) tétrahydrofurane-2-yl)-4-oxo-4,7-dihydro-3H-pyrrolo[3,2-d]pyrimidine-2-yl)isobutyramide. L'invention concerne également des polynucléotides codant pour les enzymes cétoréductases, des cellules hôtes capables d'exprimer les enzymes cétoréductases, et des méthodes d'utilisation des enzymes cétoréductases pour synthétiser le N-(9-((2R,3S,4S,5R)-4-fluoro-3-hydroxy-5-(hydroxyméthyl)tétrahydrofurane-2-yl)-6-oxo-6,9-dihydro-1H-purine-2-yl)isobutyramide.