STANDARDIZED PLANT EXTRACT FROM BIOMASS OF IN VITRO CULTURES, METHOD FOR PREPARATION AND USE THEREOF

The invention refers to standardized plant extract from biomass of in vitro cultures of Haberlea rhodopensis Friv. (HR), containing bioactive compounds and their primary secondary metabolites, containing in weight %, as follows: organic acids from 4.0 to 6.0, fatty acids from 0.5 to 1.5, amino acids from 8.0 to 12.0, sterols from 0.5 to 1.0, free phenols from 3.0 to 6.0, sugars from 45 to 55, and polyphenols from 25.0 to 35,0, with a predominant myconoside content of 70% to 96% in the polyphenolic fraction, constituting 18% to 35% of the total extract, and to a composition containing the standardized extract and glycerol as well as to a method for the the preparation of a standardized plant extract. The method according this invention, along with its optimally chosen steps, specific conditions, parameters such as temperature, duration, stirring, light, growth factors, etc. achieves both maximum volumetric productivity of the target substances and myconoside, as well as stable productivity of the plant in vitro cultures and is a reliable efficient 24/7 continuous system for production of NPs. Dependence on natural factors, limited availability and protection of HR rare wild plant populations are eliminated. The limitations posed by seasonality arid slow HR growth are also avoided by developing a renewable, ecologically method. The developed method provides alternative, renewable and sustainable sources of raw plant material necessary to obtain the target extract. The resulting extract standardized in myconoside is especially valuable with its protective action on human health and can successfully be used with its pharmacological, cosmetic effects as well as in functional foods. L'invention concerne un extrait végétal standardisé à partir de biomasse de cultures in vitro de Haberlea rhodopensis Friv. (HR), contenant des composés bioactifs et leurs métabolites secondaires primaires, contenant en % en poids, comme suit : acides organiques de 4,0 à 6,0, acides gras de 0,5 à 1,5, acides aminés de 8,0 à 12,0, stérols de 0,5 à 1,0, phénols libres de 3,0 à 6,0, sucres de 45 à 55, et polyphénols de 25.0 à 35.0, avec une teneur prédominante en myconoside de 70 % à 96 % dans la fraction polyphénolique, constituant 18 % à 35 % de l'extrait total, et à une composition contenant l'extrait standardisé et du glycérol ainsi qu'à un procédé pour la préparation d'un extrait végétal standardisé. Le procédé selon l'invention, avec ses étapes choisies de manière optimale, ses co