COMPOSITIONS THAT REDUCE TEMPLATE THREADING INTO A NANOPORE

This application discloses compositions comprising primer compounds that reduce or block deleterious threading into a nanopore of nucleic acid strands displaced by a nanopore-linked polymerase, for example during the use of a nanopore device for nucleic acid sequencing. Also disclosed are methods fo...

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Hauptverfasser:	CRISALLI, Peter, VARGAS, Adolfo, BEYER, David, DHIMAN, Kirti, KHAKSHOOR, Omid, CHAKRAVARTHY, Swathi, LANGDON, Blake, FRANKLIN, Helen, TAING, Meng, AYER, Aruna
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES DERIVATIVES THEREOF ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	This application discloses compositions comprising primer compounds that reduce or block deleterious threading into a nanopore of nucleic acid strands displaced by a nanopore-linked polymerase, for example during the use of a nanopore device for nucleic acid sequencing. Also disclosed are methods for using the compositions to reduce deleterious threading events during nanopore-based nucleic acid detection techniques, such as nanopore sequencing. L'invention concerne des compositions comprenant des composés d'amorce qui réduisent ou bloquent l'introduction néfaste dans un nanopore de brins d'acide nucléique déplacés par une polymérase liée à un nanopore, par exemple pendant l'utilisation d'un dispositif à nanopores pour le séquençage d'acides nucléiques. L'invention concerne également des procédés d'utilisation des compositions pour réduire des événements d'introduction néfaste pendant des techniques de détection d'acides nucléiques à base de nanopores, telles que le séquençage par nanopores.