MALATE DEHYDROGENASE ELECTRODE, AND PREPARATION METHOD THEREFOR AND USE THEREOF

Provided are a malate dehydrogenase electrode, and a preparation method therefor and the use thereof. The method comprises the following steps: chemically modifying a coenzyme factor small molecule NAD+ non-active part by means of a chemical means, covalently connecting the chemically modified NAD+...

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Bibliographische Detailangaben
Hauptverfasser:	YANG, Yan, MA, Yaohong, YANG, Junhui, CAI, Lei, GONG, Weili, ZHENG, Lan, MENG, Qingjun, LIU, Qingai
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided are a malate dehydrogenase electrode, and a preparation method therefor and the use thereof. The method comprises the following steps: chemically modifying a coenzyme factor small molecule NAD+ non-active part by means of a chemical means, covalently connecting the chemically modified NAD+ with a chitosan carrier to obtain an NAD+-chitosan complex, modifying a carbon nanotube on the surface of a substrate electrode, and utilizing the carbon nanotube as a substrate material for detecting NADH; electrically depositing ABTS on the electrode, and realizing NAD+ in situ regeneration by means of using the ABTS as an electron mediator; dropwise adding the NAD+-chitosan complex to realize fixation of NAD+ on the surface of the electrode; connecting a malate dehydrogenase to the chitosan carrier by means of a glutaraldehyde cross-linking action to obtain a malate dehydrogenase electrode; and combining the fixation and regeneration method of NAD+ with different types of dehydrogenases to prepare various dehydr