MEANS AND METHODS FOR SELECTIVE DOUBLE DIOL OXIDATION

The invention relates to the field of enzyme engineering and biocatalysis, in particular to alcohol oxidases and their application in the selective oxidation of diols for the production of industrially useful oxidation products thereof, such as precursors for biodegradable polyesters. Provided is a...

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Bibliographische Detailangaben
Hauptverfasser:	TRAJKOVIC, Miloš, FRAAIJE, Marco Wilhelmus, MARTIN, Caterina
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the field of enzyme engineering and biocatalysis, in particular to alcohol oxidases and their application in the selective oxidation of diols for the production of industrially useful oxidation products thereof, such as precursors for biodegradable polyesters. Provided is a method for the selective oxidation of a diol substrate, comprising subjecting a diol substrate of the formula HO-CH2-CH2-X-CH2-CH2-OH, wherein X = O, S or CH2, to a FAD-containing alcohol oxidase (AOX) enzyme of the class EC 1.1.3.13 or EC 1.1.3.17. or a mutant thereof, under conditions allowing lor the double oxidation of one hydroxyl moiety of the diol substrate into an oxidation product. Alcools oxydases et leur application dans l'oxydation sélective de diols pour la production de produits d'oxydation industriellement utiles associés, tels que des précurseurs pour polyesters biodégradables. L'invention concerne un procédé d'oxydation sélective d'un substrat diol, consistant à soumettre un substrat diol de formule HO-CH2-CH2-X-CH2-CH2-OH, dans laquelle X=O, S ou CH2, à une enzyme alcool oxydase (AOX) contenant de la FAD de la classe EC 1.1.3.13 ou EC 1.1.3.17. ou un de ses mutants, dans des conditions permettant la double oxydation d'une fraction hydroxyle du substrat diol en un produit d'oxydation.