CELL CULTURE METHOD AND APPLICATION THEREOF BASED ON HIGH-DENSITY AND CONTINUOUS INOCULATION

A cell culture method and application thereof based on high-density and continuous inoculation. The method comprises the following steps: (1) providing a cell culture, and performing resuscitation, flask shaking and expansion culture, and swing-type reaction bag expansion culture on the cell culture; (2) transferring the resuscitated and expanded cell into a last cell expansion tank so as to continue to perform expansion culture; (3) inoculating the cell in the last cell expansion tank into a culture fermenter in a high-density and continuous inoculation mode for performing fermentation culture; and (4) harvesting a target product. The cell culture method based on high-density and continuous inoculation can be used to shorten the average cell passage time of each batch, and improve the production efficiency of the cell and an expression product thereof. L'invention concerne un procédé de culture cellulaire et son application sur la base d'une inoculation continue et à haute densité. Le procédé comprend les ét