METHOD FOR PURIFYING HIGH MOLECULAR WEIGHT ADENOSINE-BASED COENZYMES BY TANGENTIAL DIAFILTRATION

The present invention relates to the field of making high molecular weight adenosine-based coenzymes available on a large scale. In particular, it relates to a method for purifying high molecular weight adenosine-based coenzymes by implementing a tangential diafiltration, or even dia-ultrafiltration...

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Bibliographische Detailangaben
Hauptverfasser:	WILLIG, Gaëlle, ALLAIS, Florent, MOUTERDE, Louis
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SEPARATION SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to the field of making high molecular weight adenosine-based coenzymes available on a large scale. In particular, it relates to a method for purifying high molecular weight adenosine-based coenzymes by implementing a tangential diafiltration, or even dia-ultrafiltration step. This method is, for example, applicable to the purification of coenzyme A disulfide ((CoAS)2), coenzyme A (CoA), nicotinamide adenine dinucleotide (NAD+), nicotinamide adenine dinucleotide phosphate (NADP+) or flavin adenine dinucleotide (FAD). La présente invention a trait au domaine de la mise à disposition à grande échelle de coenzymes à base adénosine de haut poids moléculaire. Elle concerne en particulier un procédé de purification de coenzymes à base adénosine de haut poids moléculaire mettant en œuvre une étape de dia-filtration tangentielle, voire de dia-ultrafiltration. Ce procédé est, par exemple, applicable à la purification du disulfure de coenzyme A ((CoAS)2), de la coenzyme A (CoA), du nicotinamide adénine dinucléotide (NAD+), du nicotinamide adénine dinucléotide phosphate (NADP+) ou de la flavin adénine dinucléotide (FAD).