STABILIZATION AND PRESERVATION OF IN VITRO TRANSCRIPTION REACTIONS

The disclosed subject matter relates to compositions, systems, kits, and methods that typically comprise and/or utilize a lyophilized composition comprising components for performing in vitro transcription prepared by lyophilizing an aqueous composition that includes: (i) the one or more components...

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Bibliographische Detailangaben
Hauptverfasser:	LUCKS, Julius B, CLAUER, Phillip R, LEE, Jeong W, ALAM, Khalid K, VEROSLOFF, Matthew S, JUNG, Jaeyoung K, COLLINS, James J
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The disclosed subject matter relates to compositions, systems, kits, and methods that typically comprise and/or utilize a lyophilized composition comprising components for performing in vitro transcription prepared by lyophilizing an aqueous composition that includes: (i) the one or more components for performing in vitro transcription; and (ii) a non-reducing polysaccharide at a concentration of at least about 40 mM and/or a sugar alcohol at a concentration of at least about 40 mM. In some embodiments, the lyophilized composition is prepared by lyophilizing an aqueous composition that comprises no more than 5% (v/v) glycerol. La présente invention concerne des compositions, des systèmes, des kits et des procédés comprenant habituellement et/ou utilisant une composition lyophilisée comprenant des composants pour réaliser une transcription in vitro préparée par lyophilisation d'une composition aqueuse qui comprend : (i) la ou les composants pour effectuer une transcription in vitro; et (ii) un polysaccharide non réducteur à une concentration d'au moins environ 40 mM et/ou un alcool de sucre à une concentration d'au moins environ 40 mM. Dans certains modes de réalisation, la composition lyophilisée est préparée par lyophilisation d'une composition aqueuse ne comprenant pas plus de 5% (v/v) de glycérol.