VECTOR EXPRESSING RECOMBINANT ANTIGEN BY USING CRISPR EDITING TECHNOLOGY, AND SIMULTANEOUS MULTIPLE INSERTION METHOD THEREFOR

Provided in the present invention is a method for simultaneously inserting multiple vectors expressing recombinant antigens in multiple locations of eukaryotic chromosomes by using a homology directed recombination method, which is a CRISPR technology. The present invention relates to a method for expressing a recombinant protein which can be used as an immunogen, by selecting a human cell line that can stably over-express an immunogen, when simultaneous insertion into two sites is performed, and then using the cells, and which can be effectively used for the development of recombinant immunogens and vaccines against various socially problematic viruses. La présente invention concerne un procédé d'insertion simultanée de multiples vecteurs exprimant des antigènes recombinants dans de multiples emplacements de chromosomes eucaryotes au moyen d'un procédé de recombinaison dirigée par homologie, qui est une technologie CRISPR. La présente invention concerne un procédé d'expression d'une protéine recombinante qui peut être utilisée en tant qu'immunogène, par sélection d'une lignée cellulaire humaine qui peut surexprimer de manière stable un immunogène, lorsqu'une insertion simultanée dans deux sites est effectuée, puis à l'aide des cellules, et qui peut être efficacement utilisée pour le développement d'immunogènes recombinants et de vaccins contre divers virus problématiques sur le plan social. 본 발명은 CRISPR 기술인 Homology directed recombination 방법을 통하여 진핵 세포 크로모좀의 다중 위치에 재조합 항원을 발현시키는 벡터를 동시에 다중 삽입시키는 방법을 제공한다. 동시에 두 곳에 삽입시켜서, 면역원을 안정적이고 과 발현 할 수 있는 인간 세포주를 선별한 후, 이러한 세포를 이용하여 면역원으로 사용 가능한 재조합 단백질을 발현하는 방법에 관한 것으로써, 사회적으로 문제가 되는 다양한 바이러스에 대한 재조합 면역원, 백신 개발에 유용하게 사용될 수 있다.