SUPER-RESOLUTION IMAGING METHOD AND APPARATUS, AND TERMINAL DEVICE

A super-resolution imaging method and apparatus, and a terminal device. The method comprises: exciting a fluorescent probe having a dual emission peak on a biological sample by means of an excitation light, and acting an affinity reagent onto the excited fluorescent probe (S101); irradiating, by mea...

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Bibliographische Detailangaben
Hauptverfasser:	LIU, Wufan, YANG, Zhigang, QU, Junle
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING
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Beschreibung
Zusammenfassung:	A super-resolution imaging method and apparatus, and a terminal device. The method comprises: exciting a fluorescent probe having a dual emission peak on a biological sample by means of an excitation light, and acting an affinity reagent onto the excited fluorescent probe (S101); irradiating, by means of an activated light, the fluorescent probe acted by the affinity reagent to obtain a dual-channel fluorescence intensity image (S102); respectively acquiring a short wavelength signal having a short wavelength emitting peak and a long wavelength signal having a long wavelength emitting peak in the dual-channel fluorescence intensity image (S103); selecting a fluorescence intensity image of the short wavelength signal and that of the long wavelength signal from the same frame of dual-channel fluorescence intensity image according to the respectively acquired short wavelength signal and long wavelength signal, and respectively calculating a fluorescence intensity ratio of the images to obtain N proportional fluo