ALPHA-HEMOLYSIN VARIANTS AND USES THEREOF

Described herein are variants of alpha-hemolysin having at least one mutation, such as a mutation to a positive charge. In certain examples, the mutation is selected from OK, A1 K, A1 R, D2N, S3K, D4K, D4N, K8R, N47K, E70K, S106K, E1 1 1 N, 127-131 G, D128K, K147N, V149K, E287R, M298A, or combinatio...

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Bibliographische Detailangaben
1. Verfasser:	CRAIG, Timothy K
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Described herein are variants of alpha-hemolysin having at least one mutation, such as a mutation to a positive charge. In certain examples, the mutation is selected from OK, A1 K, A1 R, D2N, S3K, D4K, D4N, K8R, N47K, E70K, S106K, E1 1 1 N, 127-131 G, D128K, K147N, V149K, E287R, M298A, or combinations thereof in the mature, wild-type alpha-hemolysin amino acid sequence. Also provided are compositions including the variants of alpha-hemolysin, nanopore assemblies including the alpha-hemolysin variants, and methods of sequencing nucleic acids incorporating the same. L'invention concerne des variants de l'alpha-hémolysine ayant au moins une mutation, telle qu'une mutation à une charge positive. Dans certains exemples, la mutation est choisie parmi OK, A1 K, A1 R, D2N, S3K, D4K, D4N, K8R, N47K, E70K, S106K, E1 1 1 N, 127-131 G, D128K, K147N, V149K, E287R, M298A, ou des combinaisons de ceux-ci dans la séquence d'acides aminés alpha-hémolysine de type sauvage mature. L'invention concerne également des compositions comprenant les variants d'alpha-hémolysine, des ensembles de nanopores comprenant les variants d'alpha-hémolysine, et des procédés de séquençage d'acides nucléiques les incorporant.