CHANNELED FIBERS IN SEPARATION OF BIOLOGICALLY ACTIVE NANOPARTICLES

A relatively fast, inexpensive, and non-destructive method for separation and isolation of biologically active nanoparticles is described. Methods include the use of solid phase separation medis such as channeled fibers in a hydrophobic interaction chromatography (HIC) protocol to isolate biological...

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Hauptverfasser:	WANG, Lei, SLONECKI, Tyler, BRUCE, Terri, MARCUS, R. Kenneth, HUANG, Sisi, POWELL, Rhonda Reigers
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	CHEMISTRY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY ORGANIC CHEMISTRY PEPTIDES PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES SEPARATION TRANSPORTING
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Zusammenfassung:	A relatively fast, inexpensive, and non-destructive method for separation and isolation of biologically active nanoparticles is described. Methods include the use of solid phase separation medis such as channeled fibers in a hydrophobic interaction chromatography (HIC) protocol to isolate biologically active nanoparticles from other components of a mixture. Biologically active nanoparticles can include natural nanoparticles (e.g., exosomes, lysosomes, virus particles) as well as synthetic nanoparticles (liposomes, genetically modified virus particles, etc.) L'invention concerne un procédé relativement rapide, peu coûteux et non destructif de séparation et d'isolement de nanoparticules biologiquement actives. Les procédés comprennent l'utilisation de milieux de séparation en phase solide tels que des fibres canalisées dans un protocole de chromatographie d'interaction hydrophobe (HIC) pour isoler des nanoparticules biologiquement actives d'autres composants d'un mélange. Les nanoparticules biologiquement actives peuvent comprendre des nanoparticules naturelles (par exemple, des exosomes, des lysosomes, des particules de virus) ainsi que des nanoparticules synthétiques (liposomes, particules de virus génétiquement modifiées, etc.)