GENOME EDITING BY POLLEN-MEDIATED TRANSFORMATION

Disclosed herein are methods of genome-editing transformation that delivers to the cell the RNA-guided endonucleases as well as the required guide RNA, without the need of tissue culture to regenerate the plant. Thus, methods as described herein enable any plant species to have its genome edited in...

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Bibliographische Detailangaben
Hauptverfasser:	LEACH, Kristen, DERVINIS, Christopher, BALMANT, Kelly Mayrink, RESENDE, Marcio Fernando, KIRST, Matias
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM ORGANIC CHEMISTRY PEPTIDES PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed herein are methods of genome-editing transformation that delivers to the cell the RNA-guided endonucleases as well as the required guide RNA, without the need of tissue culture to regenerate the plant. Thus, methods as described herein enable any plant species to have its genome edited in a process that is simple and efficient. This process utilizes genome-editing pollen cells, and delivering the required components for the editing by combining them with cell-penetrating peptides. L'invention concerne des procédés de transformation par édition de génome qui délivrent à la cellule les endonucléases guidées par l'ARN ainsi que l'ARN guide requis, sans avoir besoin d'une culture tissulaire pour régénérer la plante. Ainsi, les procédés selon l'invention permettent d'éditer le génome de n'importe quelle espèce végétale selon un procédé qui est simple et efficace. Ce procédé utilise des cellules de pollen d'édition de génome, et fournit les composants requis pour l'édition par combinaison de celles-ci avec des peptides de pénétration cellulaire.