QUANTITATIVE DETECTION AND ANALYSIS OF TARGET DNA WITH COLORIMETRIC RT-QLAMP

The present disclosure relates to real-time quantification using loop-mediated isothermal amplification, in particular real-time colorimetric reverse transcription quantitative loop-mediated isothermal amplification (RT-qLAMP). In some embodiments, RT-qLAMP is used to diagnose the presence of and al...

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Bibliographische Detailangaben
Hauptverfasser:	GAO, Weimin, CI, Shufang, MARTINEAU, Rhett, MELDRUM, Deirdre, CHAO, Shih-Hui (Joseph)
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CALCULATING CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPUTING CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES COUNTING ENZYMOLOGY IMAGE DATA PROCESSING OR GENERATION, IN GENERAL INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present disclosure relates to real-time quantification using loop-mediated isothermal amplification, in particular real-time colorimetric reverse transcription quantitative loop-mediated isothermal amplification (RT-qLAMP). In some embodiments, RT-qLAMP is used to diagnose the presence of and also quantitate the amount of Zika virus in a sample. La présente invention concerne une quantification en temps réel à l'aide d'une amplification isotherme à médiation par boucle, en particulier une amplification isotherme à médiation par boucle quantitative par transcription inverse (RT-qLAMP) colorimétrique en temps réel. Dans certains modes de réalisation, la RT-qLAMP est utilisée pour diagnostiquer la présence du virus Zika dans un échantillon et également quantifier celle-ci.