RAPID AND EFFICIENT METHOD FOR SCREENING MICROCYSTIN DEGRADING BACTERIA

Disclosed in the present invention is a rapid and efficient method for screening microcystin degrading bacteria, comprising primer pair design, polymerase chain reaction (PCR) amplification, and product detection: designing a primer pair as shown in SEQ ID NO:1 and SEQ ID NO:2; using the DNA of a mi...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	SHI, Quanliang, GU, Qing
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Disclosed in the present invention is a rapid and efficient method for screening microcystin degrading bacteria, comprising primer pair design, polymerase chain reaction (PCR) amplification, and product detection: designing a primer pair as shown in SEQ ID NO:1 and SEQ ID NO:2; using the DNA of a microcystin degrading bacteria to be screened as a template, performing PCR amplification by using the primer pair to obtain an amplification product; detecting the amplification product and completing screening of the microcystin degrading bacteria according to a detection result. La présente invention concerne un procédé rapide et efficace de criblage de bactéries dégradant la microcystine, comprenant une conception de paire d'amorces, une amplification par réaction en chaîne par polymérase (PCR) et une détection de produit : la conception d'une paire d'amorces telle que représentée dans la séquence SEQ ID NO : 1 et la séquence SEQ ID NO: 2 ; l'utilisation de l'ADN d'une bactérie dégradant la microcystine à cribler