STABLE PROTEASE VARIANTS

The present invention relates to a protease variant which is at least 90% identical to the full length amino acid sequence of a Kumamolisin AS backbone as set forth in any of SEQ ID NOs 1 - 3, while maintaining proteolytic activity, or a fragment, fraction or shuffled variant thereof maintaining pro...

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Bibliographische Detailangaben
Hauptverfasser:	SCHEIDIG, Andreas, HORN, Thomas, ELEND, Christian, MICHELS, Andreas, KRAPP, Claudia
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES BEER BIOCHEMISTRY CANDLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DETERGENT COMPOSITIONS DETERGENTS ENZYMOLOGY FATTY ACIDS THEREFROM FODDER FOODS OR FOODSTUFFS HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RECOVERY OF GLYCEROL RESIN SOAPS SOAP OR SOAP-MAKING SPIRITS THEIR TREATMENT, NOT COVERED BY OTHER CLASSES USE OF SINGLE SUBSTANCES AS DETERGENTS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a protease variant which is at least 90% identical to the full length amino acid sequence of a Kumamolisin AS backbone as set forth in any of SEQ ID NOs 1 - 3, while maintaining proteolytic activity, or a fragment, fraction or shuffled variant thereof maintaining proteolytic activity, which protease variant demonstrates altered or improved stability compared to the Kumamolisin AS wildtype as set forth in SEQ ID NO 4, or the Kumamolisin AS backbone as set forth in any of SEQ ID NOs 1 - 3. La présente invention concerne un variant de protéase qui présente une identité d'au moins 90 % avec la séquence d'acides aminés pleine longueur du squelette de la kumamolisine AS telle que définie dans l'une quelconque des SEQ ID No 1 à 3, tout en conservant son activité protéolytique, ou un fragment, une fraction ou un variant remanié de celle-ci conservant ladite activité protéolytique, où le variant de protéase présente une stabilité modifiée ou améliorée comparativement au type sauvage de la kumamolisine AS tel que défini dans SEQ ID No 4, ou au squelette de la kumamolisine AS tel que défini dans l'une quelconque des SEQ ID No 1 à 3.