METHOD FOR ESTABLISHING CD36 MUTANT GENE STABLE EUKARYOTIC EXPRESSION CELL LINE CAUSING CD36 DELETION

Provided is a method for establishing a CD36 mutant gene stable eukaryotic expression cell line causing CD36 deletion, comprising the whole process of extracting total RNA from a CD36 deletion individual and conducting reverse transcription so as to obtain CD36 cDNA of whole segment of a CD36 mutant gene open reading frame; establishing a recombinant cloning vector containing the CD36 mutant gene cDNA and an EGFP fluorescent reporter gene, transforming the recombinant cloning vector into escherichia coli, and conducting plasmid amplification and identification; and transfecting the recombinant cloning vector into CHO-K1 cells, and conducting screening and identification so as to obtain the CD36 mutant gene stable eukaryotic expression cell line. La présente invention concerne un procédé de mise en place d'une lignée cellulaire d'expression eucaryote stable à gène mutant (CD36) provoquant la délétion de CD36, comprenant le procédé complet d'extraction d'ARN total d'un individu à délétion de CD36 et la réalisat