PRODUCTION OF GLYCOSYLATED NOOTKATOL IN RECOMBINANT HOSTS

The invention relates to methods for producing glycosylated nootkatol. In particular, a recombinant host comprising a gene encoding a UDP-glycosyltransferase polypeptide capable of glycosylating nootkatol is disclosed. Glycosylation of nootkatol detoxifies nootkatol, allowing for greater production...

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Bibliographische Detailangaben
Hauptverfasser:	PROCHASSON, Philippe, SARAN, Dayal, OUSPENSKI, Alexei, HANSEN, Jorgen, ROUSSEL, Patrick, OCCHIPINTI, Laura, MALCA, Sumire
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMES ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FISHING FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J FORESTRY HUMAN NECESSITIES HUNTING HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to methods for producing glycosylated nootkatol. In particular, a recombinant host comprising a gene encoding a UDP-glycosyltransferase polypeptide capable of glycosylating nootkatol is disclosed. Glycosylation of nootkatol detoxifies nootkatol, allowing for greater production of (glycosylated-)nootkatol, a precursor of nootkatone, and therefore greater production of nootkatone. The invention also relates to methods of converting non-toxic, glycosylated nootkatol produced by a recombinant host to nootkatol, wherein the nootkatol can subsequently be converted to large quantities of nootkatone to be used in flavorings, perfumes, and/or insect repellents. L'invention concerne des procédés de production de nootkatol glycosylé. Elle se réfère en particulier à un hôte de recombinaison qui comprend un gène codant pour un polypeptide d'UDP-glycosyltransférase capable de glycosyler le nootkatol. La glycosylation du nootkatol détoxifie le nootkatol, ce qui permet d'obtenir une production accrue de nootkatol (glycosylé), un précurseur de nootkatone, et par conséquent d'obtenir une production accrue de nootkatone. L'invention concerne également des procédés de conversion de nootkatol glycosylé non toxique, produit par un hôte de recombinaison, en nootkatol, le nootkatol pouvant être transformé ultérieurement en grandes quantités de nootkatone destiné à être utilisé dans des aromatisants, des parfums et/ou des insectifuges.