POLYPEPTIDES HAVING HYDROLYTIC ACTIVITY ON 1-KESTOSE IN THE PRESENCE OF SUCROSE BUT LACKING SUCRASE (INVERTASE) ACTIVITY, POLYNUCLEOTIDES ENCODING SAME AND METHODS OF PRODUCTING AND USING SAME IN INDUSTRIAL SUCROSE PRODUCTION FROM 1-KESTOSE
The present invention relates to an efficient industrial process using kestose hydrolases to enable the use of sugar mixtures containing 1-kestose in the industrial production of sucrose (as crystallized sugar). The present invention method provides the conversion of 1-kestose into sucrose and fruct...
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| creator | GOUVEA, Iuri Estrada CAMILO, Cesar Moises FINGUERUT, Jaime COOK, Michael |
| description | The present invention relates to an efficient industrial process using kestose hydrolases to enable the use of sugar mixtures containing 1-kestose in the industrial production of sucrose (as crystallized sugar). The present invention method provides the conversion of 1-kestose into sucrose and fructose in a sugar solution, containing kestose and more than 10 mM sucrose (3g/L), wherein said method comprises the enzymatic hydrolysis (preferably using 1-FEH enzymes - EC 3.2.1.153) of 1-kestose. The present invention also seeks to provide a method of producing a polypeptide having 1-Kestose Hydrolase activity and a composition comprising the polypeptide herein.
La présente invention concerne un procédé industriel efficace utilisant des kestose hydrolases pour permettre l'utilisation de mélanges de sucres contenant du 1-kestose dans la production industrielle de saccharose (en tant que sucre cristallisé). Le procédé de la présente invention comprend la conversion du 1-kestose en saccharose et en fructose dans une solution de sucres contenant du kestose et plus de 10 mM de saccharose (3 g/l), ledit procédé comprenant l'hydrolyse enzymatique du 1-kestose (de préférence à l'aide d'enzymes 1-FEH - EC 3.2.1.153). La présente invention concerne également un procédé de production d'un polypeptide présentant une activité 1-kestose hydrolase et une composition comprenant ledit polypeptide en son sein. |
| format | Patent |
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La présente invention concerne un procédé industriel efficace utilisant des kestose hydrolases pour permettre l'utilisation de mélanges de sucres contenant du 1-kestose dans la production industrielle de saccharose (en tant que sucre cristallisé). Le procédé de la présente invention comprend la conversion du 1-kestose en saccharose et en fructose dans une solution de sucres contenant du kestose et plus de 10 mM de saccharose (3 g/l), ledit procédé comprenant l'hydrolyse enzymatique du 1-kestose (de préférence à l'aide d'enzymes 1-FEH - EC 3.2.1.153). La présente invention concerne également un procédé de production d'un polypeptide présentant une activité 1-kestose hydrolase et une composition comprenant ledit polypeptide en son sein.</description><language>eng ; fre</language><subject>APPARATUS SPECIALLY ADAPTED THEREFOR ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PRODUCTION OF SUCROSE ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; SUGAR INDUSTRY ; VINEGAR ; WINE</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170518&DB=EPODOC&CC=WO&NR=2017079818A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170518&DB=EPODOC&CC=WO&NR=2017079818A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>GOUVEA, Iuri Estrada</creatorcontrib><creatorcontrib>CAMILO, Cesar Moises</creatorcontrib><creatorcontrib>FINGUERUT, Jaime</creatorcontrib><creatorcontrib>COOK, Michael</creatorcontrib><title>POLYPEPTIDES HAVING HYDROLYTIC ACTIVITY ON 1-KESTOSE IN THE PRESENCE OF SUCROSE BUT LACKING SUCRASE (INVERTASE) ACTIVITY, POLYNUCLEOTIDES ENCODING SAME AND METHODS OF PRODUCTING AND USING SAME IN INDUSTRIAL SUCROSE PRODUCTION FROM 1-KESTOSE</title><description>The present invention relates to an efficient industrial process using kestose hydrolases to enable the use of sugar mixtures containing 1-kestose in the industrial production of sucrose (as crystallized sugar). The present invention method provides the conversion of 1-kestose into sucrose and fructose in a sugar solution, containing kestose and more than 10 mM sucrose (3g/L), wherein said method comprises the enzymatic hydrolysis (preferably using 1-FEH enzymes - EC 3.2.1.153) of 1-kestose. The present invention also seeks to provide a method of producing a polypeptide having 1-Kestose Hydrolase activity and a composition comprising the polypeptide herein.
La présente invention concerne un procédé industriel efficace utilisant des kestose hydrolases pour permettre l'utilisation de mélanges de sucres contenant du 1-kestose dans la production industrielle de saccharose (en tant que sucre cristallisé). Le procédé de la présente invention comprend la conversion du 1-kestose en saccharose et en fructose dans une solution de sucres contenant du kestose et plus de 10 mM de saccharose (3 g/l), ledit procédé comprenant l'hydrolyse enzymatique du 1-kestose (de préférence à l'aide d'enzymes 1-FEH - EC 3.2.1.153). La présente invention concerne également un procédé de production d'un polypeptide présentant une activité 1-kestose hydrolase et une composition comprenant ledit polypeptide en son sein.</description><subject>APPARATUS SPECIALLY ADAPTED THEREFOR</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PRODUCTION OF SUCROSE</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>SUGAR INDUSTRY</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNTr1OwzAQzsKAgHc4iQUkKjV0aBmNfcGnJj7LPgdlqipkJgSVyoPzCNj8hJXpTt__afPhuZ88eiGDEawayT2AnUwosJAGpYVGkgnYQbvYYhSOCORALIIPGNFpBO4gJh0qdZ8EeqW3NadiqmBX5EYMUt7rOfAGarNLukf-Li9JbL5sakBQzsCAYtnEGu8Dm1Ssha5MirOwbCFnUpRAqp9n_OrL7C7w8Lf9vDl53r8c88XPPWsuOxRtF_nwtsvHw_4pv-b33SPfLtv1cn23aTeqXf1P9Qkz8Vwj</recordid><startdate>20170518</startdate><enddate>20170518</enddate><creator>GOUVEA, Iuri Estrada</creator><creator>CAMILO, Cesar Moises</creator><creator>FINGUERUT, Jaime</creator><creator>COOK, Michael</creator><scope>EVB</scope></search><sort><creationdate>20170518</creationdate><title>POLYPEPTIDES HAVING HYDROLYTIC ACTIVITY ON 1-KESTOSE IN THE PRESENCE OF SUCROSE BUT LACKING SUCRASE (INVERTASE) ACTIVITY, POLYNUCLEOTIDES ENCODING SAME AND METHODS OF PRODUCTING AND USING SAME IN INDUSTRIAL SUCROSE PRODUCTION FROM 1-KESTOSE</title><author>GOUVEA, Iuri Estrada ; CAMILO, Cesar Moises ; FINGUERUT, Jaime ; COOK, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2017079818A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2017</creationdate><topic>APPARATUS SPECIALLY ADAPTED THEREFOR</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PRODUCTION OF SUCROSE</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>SUGAR INDUSTRY</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>GOUVEA, Iuri Estrada</creatorcontrib><creatorcontrib>CAMILO, Cesar Moises</creatorcontrib><creatorcontrib>FINGUERUT, Jaime</creatorcontrib><creatorcontrib>COOK, Michael</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>GOUVEA, Iuri Estrada</au><au>CAMILO, Cesar Moises</au><au>FINGUERUT, Jaime</au><au>COOK, Michael</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>POLYPEPTIDES HAVING HYDROLYTIC ACTIVITY ON 1-KESTOSE IN THE PRESENCE OF SUCROSE BUT LACKING SUCRASE (INVERTASE) ACTIVITY, POLYNUCLEOTIDES ENCODING SAME AND METHODS OF PRODUCTING AND USING SAME IN INDUSTRIAL SUCROSE PRODUCTION FROM 1-KESTOSE</title><date>2017-05-18</date><risdate>2017</risdate><abstract>The present invention relates to an efficient industrial process using kestose hydrolases to enable the use of sugar mixtures containing 1-kestose in the industrial production of sucrose (as crystallized sugar). The present invention method provides the conversion of 1-kestose into sucrose and fructose in a sugar solution, containing kestose and more than 10 mM sucrose (3g/L), wherein said method comprises the enzymatic hydrolysis (preferably using 1-FEH enzymes - EC 3.2.1.153) of 1-kestose. The present invention also seeks to provide a method of producing a polypeptide having 1-Kestose Hydrolase activity and a composition comprising the polypeptide herein.
La présente invention concerne un procédé industriel efficace utilisant des kestose hydrolases pour permettre l'utilisation de mélanges de sucres contenant du 1-kestose dans la production industrielle de saccharose (en tant que sucre cristallisé). Le procédé de la présente invention comprend la conversion du 1-kestose en saccharose et en fructose dans une solution de sucres contenant du kestose et plus de 10 mM de saccharose (3 g/l), ledit procédé comprenant l'hydrolyse enzymatique du 1-kestose (de préférence à l'aide d'enzymes 1-FEH - EC 3.2.1.153). La présente invention concerne également un procédé de production d'un polypeptide présentant une activité 1-kestose hydrolase et une composition comprenant ledit polypeptide en son sein.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | APPARATUS SPECIALLY ADAPTED THEREFOR BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PRODUCTION OF SUCROSE PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGAR INDUSTRY VINEGAR WINE |
| title | POLYPEPTIDES HAVING HYDROLYTIC ACTIVITY ON 1-KESTOSE IN THE PRESENCE OF SUCROSE BUT LACKING SUCRASE (INVERTASE) ACTIVITY, POLYNUCLEOTIDES ENCODING SAME AND METHODS OF PRODUCTING AND USING SAME IN INDUSTRIAL SUCROSE PRODUCTION FROM 1-KESTOSE |
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