UNIVERSAL PRIMING MIXTURE FOR AMPLIFICATION OF INFLUENZA VIRAL GENE SEGMENTS FOR DIAGNOSTIC APPLICATIONS

Provided herein is a multiplexed RT-PCR based assay for amplification multiple whole gene segments of influenza A and B. Accordingly, various oligonucleotide primers are disclosed, including for use in a multiplex RT-PCR process for influenza detection and characterization. The primers and instructi...

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Bibliographische Detailangaben
Hauptverfasser:	TAYLOR, Amber W, TENENT, Erica Dawson, ROWLEN, Kathy L
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided herein is a multiplexed RT-PCR based assay for amplification multiple whole gene segments of influenza A and B. Accordingly, various oligonucleotide primers are disclosed, including for use in a multiplex RT-PCR process for influenza detection and characterization. The primers and instructions for use may be provided in the form of a kit. Applications for the primers and related methods include as a clinical virology tool, epidemiological and surveillance tool, as well as in animal surveillance testing for influenza viruses. La présente invention concerne un essai à base de RT-PCR multiplexe pour l'amplification de multiples segments de gène entier de la grippe A et B. En conséquence, diverses amorces oligonucléotidiques sont décrites, y compris pour une utilisation dans un procédé RT-PCR multiplexe pour la détection et la caractérisation de la grippe. Les amorces et des modes d'emploi peuvent être prévus sous la forme d'un kit. Des applications pour les amorces et les procédés associés comportent une utilisation comme outil virologique clinique et comme outil épidémiologique et de surveillance, ainsi que dans des essais de surveillance animale pour les virus de la grippe.